Mattair Springs has two marked horse trails. The perimeter trail is marked with white diamonds and the interior trail is marked with yellow diamonds; the blue diamonds are a shortcut back to the parking area.
Visitors may also enjoy biking, hiking, and wildlife viewing on administrative roads and designated trails. The Florida National Scenic Trail traverses the Camp Branch tract along the river.
Travel north on US 129, turn right on CR 136A; travel east 1 mile to 85th Road; travel north 1 mile, turn right on 75th Drive. The entrance to the Mattair Springs tract is approximately 1 mile on the left.
Travel west on CR 25A, turn left past the Camp Branch road sign onto an unnamed road; follow road approximately 1 mile to the parking area.